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Arabidopsis knock out mutants of GABA metabolism and their response to different growth
conditions |
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Frank Ludewig |
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Botanisches Institut, University of Cologne, Gyrhofstr. 15, D-50931
Cologne, Germany |
| *email:
frank.ludewig@uni-koeln.de
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Arabidopsis mutants defective in genes of the
g-aminobutyric acid (GABA) pathway were isolated and analysed regarding their growth behaviour on agar plates
containing different nitrogen sources. Several carbonic and amino acids including GABA were monitored under
these conditions using GC/MS. GABA-transaminase knock out plants were hypersensitive against GABA when grown
on agar plates.In contrast to WT plants, mutants did not grow on plates containing high concentrations of GABA
as sole nitrogen source. Strikingly, growth of both, WT and mutant plants was much less inhibited without any
nitrogen in the medium indicating that GABA sensing differs between Arabidopsis WT and mutant plants,
i.e. a situation where GABA either can or cannot be used. |
