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Expression and localization of Arabidopsis synaptotagmins |
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Boris
Voigt1,*, Molly Craxton2, Bazbek Davletov2, Patrick J.
Hussey3, Diedrik Menzel1, František Baluška1 |
1 Institute of
Molecular and Cellular Biology, University of Bonn, Bonn, Germany
2 MRC Laboratory of
Molecular Biology, Cambridge, UK
3 Integrative Cell Biology Laboratory, University of
Durham, Durham, UK |
| *email:
bvoigt@uni-bonn.de
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Synaptotagmins are known Ca2+ dependent triggers of
exocytosis and endocytosis in animals with nervous system. Sequence analysis of various animal and plant
genomes revealed the presence of synaptotagmin genes in all animals and land plants, but there is no evidence
of synaptotagmin genes in unicellular organisms or those with simple forms of multicellularity. Synaptotagmins
have a common structure, a N-terminal transmembrane sequence is followed by a linker and two distinct C2
domains, C2A and C2B. These two domains are known Ca2+ binding domains, but there is nothing more
known about the function of the proteins in plants. To get more information about the 6 members of the
Arabidopsis synaptotagmin gene family, we are investigating expression patterns by promotor-GUS fusions and
cellular localizations by GFP-fusions as well as antibody labelings. Preliminary data suggest that the members
of the synaptotagmin gene family are differently expressed, but show similar cellular
localization. |
