| Differential display proteomic analysis of Picea meyeri pollen germination and pollen tube growth after actin depolymerization by latrunculin B |
| Chen Y, Chen T, Shen S, Zheng M, Guo Y, Lin J, Baluška F, Šamaj J |
| Plant J 47, 174-195 (2006) |
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| To investigate roles of the actin cytoskeleton in the growth of pollen tube of Picea meyeri Rehd. et Wils., we used actin polymerization inhibitor latrunculin B (LATB) under quantitatively controlled conditions. At low concentrations, LATB inhibited the actin cytoskeleton polymerization in the growing pollen tube, which rapidly inhibited tip growth. Proteomic approach was used to analyze protein expression profile changes during pollen germination and subsequent pollen tube development with disturbed organization of the actin cytoskeleton. Two-dimensional electrophoresis and staining with Coomassie Brilliant Blue revealed nearly 600 protein spots. A total of 84 of these were differentially displayed at different hours with varying doses of LATB, and 53 up-regulated or down-regulated proteins were identified by mass spectrometry. These proteins were grouped into distinct functional categories including signaling, actin cytoskeleton organization, cell expansion and carbohydrate metabolism. Moreover, actin disruption affected morphology of Golgi stacks, mitochondria and amyloplasts along with a differential expression of proteins involved in their functions. These findings provide new insights into the multifaceted mechanism of the actin cytoskeleton functions and its interaction with signaling, cell expansion machinery and energy-providing pathways. |
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