| Steedman‘s wax for F-actin visualization |
| Vitha S, Baluška F, Jásik J, Volkmann D, Barlow PW |
| In: Actin: a Dynamic Framework for Multiple Plant Cell Functions, CJ Staiger, F Baluška, D Volkmann, PW Barlow (eds), Kluwer Academic Publishers, Dordrecht, The Netherlands , 619-636 (2000) |
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| Actin filaments are visualised by means of indirect immunofluorescence in plant tissues that were fixed in formaldehyde, embedded in low-melting polyester wax and sectioned on a microtome. The technique described here avoids usage of detergents and organic solvents and is also compatible with immunolocalization of many other antigens. |
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